Post-Assembly Functionalization of Maleimide-Derivatized Lipid Nanoparticles with Peptide and Antibody Ligands via Thiol-Maleimide Conjugation Chemistry
Technical Bulletins

Share

Post-Assembly Functionalization of Maleimide-Derivatized Lipid Nanoparticles with Peptide and Antibody Ligands via Thiol-Maleimide Conjugation Chemistry

Introduction

Lipid nanoparticles (LNPs) are versatile carriers for nucleic acid delivery, yet their natural tropism toward the liver limits extra-hepatic applications. To overcome this limitation, surface modification with active targeting ligands has emerged as an effective strategy.

This technical note describes a unified approach for post-assembly functionalization of maleimide-derivatized LNPs using thiol-maleimide chemistry to attach two ligands with different molecular sizes:

  • CREKA peptide (~0.7 kDa), a tumor-homing pentapeptide that binds fibrin-fibronectin complexes.
  • Anti-CD3 monoclonal antibody (~150 kDa), an immunoglobulin targeting the CD3 receptor on T-cells.

By comparing both conjugation strategies, this study demonstrates the robustness and adaptability of the thiol-maleimide platform across ligands with vastly different molecular weights and structural complexities.

Want to Learn More?

Download Content

Phosphorex LLC is committed to protecting and respecting your privacy, and we'll only use your personal information to administer your account and to provide the products and services you requested from us. From time to time, we would like to contact you about our products and services, as well as other content that may be of interest to you. If you consent to us contacting you for this purpose, please tick below to say how you would like us to contact you:

You can unsubscribe from these communications at any time. For more information on how to unsubscribe, our privacy practices, and how we are committed to protecting and respecting your privacy, please review our Privacy Policy.

By clicking submit below, you consent to allow Phosphorex LLC to store and process the personal information submitted above to provide you the content requested.